Exhaustive aerobic exercise increases oxidative stress in trained runner runners
Abstract
Energy metabolism is the major producer of reactive oxygen species (ROS). Thus, intense exercise increases the production of ROS, which can cause oxidative stress. this study aimed evaluate the oxidative stress caused by an exhaustive exercise session in trained runners. Eleven runners were submitted to exhaustive treadmill exercise protocol. Blood was collected before, immediately after and 24 hours after the exercise bout. Malondialdehyde (MDA) concentration in plasma was used as oxidative damage marker. MDA concentration was evaluated by high performance liquid chromatography. To evaluate the changes in antioxidant systems, the activity of the superoxide dismutase (SOD) and catalase (CAT) enzymes, and the concentration of non-protein thiols were evaluated by spectrophotometry. The concentration of MDA was higher 24 hours after the exercise in relation to the post-exercise period. CAT activity was greater 24 hours after the exercise, in relation to the other periods. SOD activity immediately after exhaustion was lower than baseline value. The concentration of non-protein thiols was lower in the periods immediately after and 24 hours after the exhaustive effort. It is concluded that exhaustive exercise raises the oxidative stress of trained runners, however changes in markers can not be completely verified immediately after exercise, being more pronounced 24 hours after the exhaustive exercise.
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