Relationships of the inflammatory and oxidative state with adaptation in rats submitted to an overtraining induction protocol
Abstract
The objective of this manuscript was to investigate the relationship among inflammation and oxidation with adaptation in rats submitted to an overtraining-inducing protocol. Our protocol lasted 11 weeks, considering 5x/week treadmill sessions, 60minutes/session, considering training sessions since 1x/day until 4x/day. We analysed hepatic and muscular citokynes concentration including Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 6 and Interleukin 10, added to Glutamine and Glutamate muscular concentration. Indeed, we analysed serum concentrations of C-Reactive Protein, Albumin, Uric Acid, Ferric Reducing Ability of Plasma, Creatine Kinase, Urea, Total Proteins, Creatinine and Hemogram. Performance and biomarkers were analysed after 9 weeks (groups Trained [Tr] and Control [CO]) and 11 weeks of training (group Functional Overreaching [FOR]). Our results showed significant increase in performance of FOR in relation to Tr and CO groups (p<0,05). Group FOR still showed a greater anti-inflammatory/antioxidant serum and muscular status in relation to Tr and CO groups (p<0,05). At the same time, FOR showed a greater hepatic pro-inflammatory status in relation to CO (p<0,05), added to a decrease in Glutamine/Glutamate ratio in relation to Tr (p<0,05) and a decrease in hematocrit in relation to Tr and CO groups (p<0,05). Our results suggest that rats from group FOR were well-adapted to the training protocol, considering controlled levels of muscle damage and systemic inflammation, added to a greater performance status after 11 weeks of training program. Adding to this, FOR showed a better balance considering anti-inflammatory/inflammatory and antioxidante/oxidant ratios.
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