Comparison between two techniques of regional sweat collection during exercise in the heat
Abstract
Introduction: The evaluation of sweat electrolyte concentration was originally used to cystic fibrosis diagnoses. Recently, this evaluation has been used to determine athletes’ electrolyte losses during exercise in the heat. For those determinations, the sweat is usually collected by two techniques: filter paper and patches. Objective: To compare both techniques of sweat collection regarding sweat volume and sweat sodium [Na+] and chloride [Cl-] concentrations. Materials and Methods: Eleven adolescents, nine boys and two girls, took part in this study. The sweat was collected simultaneously by the two techniques. The patches and the filter paper were placed on both sides of the dorsal region. Participants cycled in the heat (35°C and 60% RH) of a climaticchamber for 30 min at 50 to 60% of their predetermined maximal workload. Sweat volume was determined by the changes in both patches and filter papers after the exercise in the heat session. Sweat [Na+] was determined by flame photometry and sweat [Cl-] bycolorimetric assay. Results: The sweat volume absorbed by the patches was higher than that absorbed by the filter paper: 0.51 ± 0.196 ml e 0.37 ± 0.013 ml (p<0.05), respectively. Sweat [Na+] was significantly higher in the filter paper, 54.2 ± 21.6 mEq/l, than that in the patches, 42.5 ± 16.9 mEq/l (p<0.05). Sweat [Cl-] was also higher in the filter paper, 53.7 ± 20.2 mEq/l, than that in the patches, 38.7 ± 17.9 mEq/l (p<0.05). Conclusion: The patches seemed to be more adequate for sweat collection duringexercise due to the increased absorption and for showing a sweat [Na+] and [Cl-] closer to those reported in the literature.
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