Oxidative stress levels and muscle cell changes in young tennis players during a competitive period
Abstract
Exercise induces an increase in oxygen consumption and energy demand. The increase in oxygen consumption leads to increased production of reactive oxygen species (ROS). Depending on your concentration, ROS react with cellular structures such as membranes, components and elements of the mitochondrial DNA, which can lead to death of DNA (apoptosis). The increased athletic performance in recent years is directly related to research that modulate the antioxidant capacity of athletes, reducing the potential oxidative cell. The aim of this study was to investigate the behavior plasma levels of cellular changes through analyses of lipid peroxidation (TBARS) and leakage of creatine kinase (CK) and the modulation of antioxidant enzyme catalase (CAT) and total shulfhydryl groups (TSG) in erythrocytes of young males tennis players in a competitive period. Three moments were analyzed: Analysis 1: (TBARS: 3.27±0.91 nmol/mL; CK: 235±37 U/L; CAT: 0,53±0,08 k/gHb/min; TSG: 555±49 µM); Analysis 2 (TBARS: 5.3±1.15 nmol/mL; CK: 476±34 U/L; CAT: 0.56±0.09k/gHb/min; TSG: 560±56 µM); Analysis 3 (TBARS: 4.6±0.54 nmol/mL; CK: 340±17 U/L; CAT: 0.5 ±0.07 k/gHb/min; TSG: 547±38 M). The values of antioxidants remained elevates compared to subjects healthy non-athletes, demonstrating adaptation to the training; however, there were significant increases in Analysis 2 and Analysis 3 for TBARS and CK in relationship to Analysis 1 (p<0.001; p<0.05, respectively) and high correlationship index (c = 0.952). In conclusion, we suggest that the use of dosages oversimplified, as the plasma CK, may indicate the extent of oxidative stress levels in muscle cells, as well as defining levels of security for determining the modulation of training loads.
References
-Aebi, H. Catalase. In: Packer L, (ed). Methods in enzymology. Florida: Academic Press, 1984.
-Alessio, H.M.; Goldfarb, A.H. Lipid peroxidation and scavenger enzymes during exercise: adaptative response to training. J Appl Physiol. Vol. 64. p. 1333-1336. 1988.
-Antunes Neto, J.M.F.; Ferreira, D.C.B.G.; Reis, I.C.; Calvi, R.G.; Rivera, R.J.B. Manutenção de microlesões celulares e respostas adaptativas a longo prazo no treinamento de força. Brazilian Journal of Biomotricity. Vol. 1. p. 87-102. 2007.
-Antunes Neto, J.M.F.; Paula, L.B. Índices de estresse oxidativo em sujeitos com diferentes níveis de composição corporal e aderência a prática de atividade física. Brazilian Journal of Biomotriciy. Vol. 5. p. 117-131. 2011.
-Antunes Neto, J.M.F.; Pereira-da-Silva, L.; Macedo, D.V. Biomarcadores de estresse oxidativo: novas possibilidades de monitoramento em treinamento físico. R Bras Ci e Mov. Vol. 12. p. 73-80. 2005.
-Antunes Neto, J.M.F.; Toyama, M.H.; Carneiro, E.M.; Boschero, A.C.; Pereira-da-Silva, L.; Macedo, D.V. Circulating leukocyte heat shock protein (HSP70) and oxidative stress markers in rats after a bout of exhaustive exercise. Stress. Vol. 9. p. 107-115. 2006.
-Beutler, E. Red cell metabolism. In: A manual of biochemical methods. London: Grune & Stratton Publishers, 1975.
-Chance, B.; Sies, H.; Boveris, A. Hydroperoxide metabolism in mammalian organs. Physiol Rev. Vol. 59. p. 527-605. 1979.
-Cruzat, V.F.; Rogero, M.M.; Borges, M.C.; Tirapegui, J. Aspectos atuais sobre estresse oxidativo, exercícios físicos e suplementação. Rev Bras Med Esporte. Vol. 13. p. 336-342. 2007.
-Davies, K.J.A.; Quintanilha, A.T.; Brook, G.A.; Packer, L. Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun. Vol. 107. p. 1198-1205. 1982.
-De Zwart, L.L.; Meerman, J.H.N.; Commandeur, J.N.M. Biomarkers of free radical damage applications in experimental animals and in humans. Free Rad Biol Med. Vol. 26. p. 202-226. 1999.
-Faure, P.; Lafond, J.L. Measurement of plasma sulphydryl and carbonyl groups as a possible indicator of protein oxidation. In: Favier, A.E. et al. (Eds.). Analysis of free radicals in biological systems. Basel: Birkhäuser Verlag, 1995. p. 237-48.
-Fridén, J.; Lieber, R.L. Structural and mechanical basis of exercise-induced muscle injury. Med Sci Sports Exerc. Vol. 24. p. 521-530. 1992.
-Fry, A.C. The role of training intensity in resistance exercise-overtraining and overreaching. In: Overtraining in Sport. Kreider, R. B.; e colaboradores(editors). Champaign, IL: Human Kinetics, p. 107-130, 1998.
-Halliwell, B.; Gutteridge, J.M.C. Free radicals in biology and medicine. 2nd ed. Oxford: Clarendon, 1989.
-Hawkins, P.T.; Poyner, D.R.; Jackson, T.R.; Letcher, A.J.; Lander, D.A.; Irvine, R.F. Inhibition of iron-catalysed hydroxyl radical formation by inositol polyphosphates: a possible physiological function for myo-inositol hexakisphosphate. Biochem J. Vol. 294. p. 929-934. 1993.
-Janero, D.R. Malondialdeyde and thiobarbithuric acid reactive as diagnostic indices of lipid peroxidation and peroxidative tissue. Free Rad Biol Med. Vol. 4. p. 515-540. 1990.
-Kosenko, E.A.; Kaminsky, Y.G.; Stavrovskaya, I.G.; Sirota, T.V.; Kondrashova, M.N. The stimulatory effect of negative air ions and hydrogen peroxide on the activity of superoxide dismutase. FEBS Letters. Vol. 410. p. 309-312. 1997.
-Lieber, R.L.; Thornell, L.E.; Fridén, J. Muscle cytoskeletal disruption occurs within the first 15 min of cyclic eccentric contraction. J Appl Physiol. Vol. 80. p. 278-284. 1996.
-Olinescu, R.; Talaban, D.; Nita, S.; Mihaescu, G. Comparative study of the presence of oxidative stress in sportsmen in competition and aged people, as well as the preventive effect of selenium administration. Rom J Intern Med. Vol. 33. p. 47-54. 1995.
-Power, S.K.; Criswell, D.; Lawler, J.; Ji, L.L.; Martim, D.; Herb. R.A.; e colaboradores. Influence of exercise and fiver type on antioxidante enzyme activity in rat skeletal muscle. Am J Physiol. Vol. 266. p. R375-R380. 1994.
-Schneider, C.D.E.; Oliveira, A.R. Radicais livres de oxigênio e exercício: mecanismos de formação e adaptação ao treinamento físico. Rev Bras Med Esporte. Vol. 10. p. 190-197. 2004.
-Selamoglu, S.; Turgay, F.; Kayatekin, B.M.; Gonenc, S.; Yslengen, C. Aerobic and anaerobic training effects on the antioxidant enzymes of the blood. Acta Physiol Hung. Vol. 87. p. 267-273. 2000.
-Tauler, P.; Gimeno, I.; Aguiló, A.; Guix, M.P.; Pons, A. Regulation of erytrocyte antioxidant enzyme activities in athletes during competition and short-term recovery. Pflügers Arch. Vol. 438. p. 782-787. 1999.
-Verkhoshanski, Y.V. Treinamento desportivo: teoria e metodologia. 1. ed. Porto Alegre: Artmed, 2000.
-Yagi, K.A. A simple fluorimetric assay for lipoperxide in blood plasma. Biochem Med. Vol. 14. p. 212-216. 1976.
-Yu, B.P. Cellular defenses against damage from reactive oxygen species. Physiol Rev. Vol. 74. p. 139-162. 2004.
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